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Soon after incorporating streptavidin-coated microparticles, the intricate is certain to the reliable stage by means of interaction with biotin and streptavidin. The reaction combination is aspirated in to the measuring cell, where the microparticles are magnetically captured onto the area with the electrode. Unbound substances are taken off, and also a voltage is placed on the electrode.[31]
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A photomultiplier steps the emitted chemiluminescence, and benefits are established through a calibration curve created by a two-place calibration as well as a learn curve (5-issue calibration) supplied by means of the reagent barcode.
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